Difference between revisions of "Part:BBa I739203"
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| − | In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], a MCS harbouring a SpeI site was introduced by PCR. This is the only difference | + | In order to make this plasmid compatible with the [https://parts.igem.org/wiki/index.php/Assembly:Standard_assembly BioBrick Standard assembly process], a MCS harbouring a SpeI site was introduced by PCR. This MCS is the only difference between pCKMCS1 and pCK01:<br> |
[[Image:PCK01-2.jpg]] | [[Image:PCK01-2.jpg]] | ||
Revision as of 20:32, 22 October 2007
No part name specified with partinfo tag.
Plasmid pCK01MCS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.
In order to make this plasmid compatible with the BioBrick Standard assembly process, a MCS harbouring a SpeI site was introduced by PCR. This MCS is the only difference between pCKMCS1 and pCK01:
Purpose
This plasmid was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and contains...
References
[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000
Sequence and Features
No part name specified with partinfo tag.