Difference between revisions of "Part:BBa K2475001"

Revision as of 21:57, 25 October 2017

Sup 35 Prion Domain fused to Cyan Fluorescent Protein (PrD-CFP)

This composite part consists of the Sup35 aggregation domain fused with CFP (cyan fluorescent protein), which is a fluorescent tag with a theoretical excitation peak at 430 nm and emission peak at 485 nm. This was used, in conjunction with PrD-YFP, in Förster resonance energy transfer (FRET) experiments to test if/how the aggregation domain affects the energy transfer from CFP to YFP in PSI+ (aggregate state) and psi- (soluble state). Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 715
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2475001 short</partinfo>
-This composite part consists of the Sup35 aggregation domain fused with yellow fluorescent protein (YFP), which is a fluorescent tag with a theoretical excitation peak at 514 nm and emission peak at 535 nm. This was used, in conjunction with PrD-CFP, in Förster resonance energy transfer (FRET) experiments test if/how the aggregation domain affects the energy transfer from CFP to YFP  expression in aggregate state  (PSI+) and soluble state (PSI-).
+This composite part consists of the Sup35 aggregation domain fused with CFP (cyan fluorescent protein), which is a fluorescent tag with a theoretical excitation peak at 430 nm and emission peak at 485 nm. This was used, in conjunction with PrD-YFP, in Förster resonance energy transfer (FRET)  experiments  to  test if/how the aggregation domain affects the energy transfer from CFP to YFP in PSI+ (aggregate state) and psi- (soluble state).
 <!-- Add more about the biology of this part here
 ===Usage and Biology===