Difference between revisions of "Part:BBa I739203"
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<partinfo>BBa_I739201 short</partinfo> | <partinfo>BBa_I739201 short</partinfo> | ||
| − | Plasmid | + | Plasmid pCK01MS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV. |
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[[Image:Pck01 Andy.jpg|350px]] | [[Image:Pck01 Andy.jpg|350px]] | ||
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| + | In contrast to the original cloning vector, we removed an intrinsic SpeI site by PCR and also introduced the following MCS: | ||
[[Image:PCK01-2.jpg]] | [[Image:PCK01-2.jpg]] | ||
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=== References === | === References === | ||
Revision as of 20:17, 22 October 2007
No part name specified with partinfo tag.
Plasmid pCK01MS1 is based on pCK01 [1], a low-copy cloning vector that confers chloramphenicol resistance (CmR) and harbours an oriV.
In contrast to the original cloning vector, we removed an intrinsic SpeI site by PCR and also introduced the following MCS:
References
[1] Fernández S, de Lorenzo V, Pérez-Martín J "Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains", Mol Microbiol. 16(2):205-13, 2000
Sequence and Features
No part name specified with partinfo tag.