Difference between revisions of "Part:BBa K2235009:Experience"

Revision as of 18:27, 25 October 2017

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Molecular cloning

Ligation of sialidase composite insert into pSB1C3

The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the double digestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.

Figure 1

User Reviews

UNIQ9069b907dc093d6f-partinfo-00000000-QINU UNIQ9069b907dc093d6f-partinfo-00000001-QINU

@@ Line 8: / Line 8: @@
 '''Ligation of sialidase composite insert into pSB1C3'''
-The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
+The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the double digestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
+[[File:SiaComp_1.png|600px|thumb|left|Figure 1]]
 ===User Reviews===