Difference between revisions of "Part:BBa K2368028"
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<h1>Introduction</h1>
 
<h1>Introduction</h1>
 
<partinfo>BBa_K2368028 ort</partinfo>
 
<partinfo>BBa_K2368028 ort</partinfo>
 
<p> Gα plays an important role in<i> Saccharomyces Cerevisiae CEN.PK2-1C </i>MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream. </p>
 
<p> Gα plays an important role in<i> Saccharomyces Cerevisiae CEN.PK2-1C </i>MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream. </p>
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[[File:Ga-1.png|945px|加载失败时候的说明文字]]
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<p style="text-align: center"> Fig.1 The structure of Gα</p>
 
<h1>Design</h1>
 
<h1>Design</h1>
 
<p> Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.</p>
 
<p> Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.</p>
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https://static.igem.org/mediawiki/2017/4/41/G-1.png
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[[File: G-1.png|945px|加载失败时候的说明文字]]
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<p> Fig.1 The schematic diagram of Modified Gα</p>
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<p style="text-align: center"> Fig.2 The schematic diagram of Modified Gα</p>
 
<h1>Experiment</h1>
 
<h1>Experiment</h1>
 
<p> The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.</p>
 
<p> The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.</p>
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https://static.igem.org/mediawiki/2017/1/18/G-2.png
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[[File: G-2.png|945px|加载失败时候的说明文字]]
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<p> The electrophoresis of the positive result of Gα.</p>
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<p style="text-align: center"> Fig.3 The electrophoresis of the positive result of Gα.</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 18:03, 25 October 2017

Introduction

BBa_K2368028 ort Not understood

Gα plays an important role in Saccharomyces Cerevisiae CEN.PK2-1C MAPK pathway. In the yeast endogenous metabolic pathway, the STE2’s conformation changes and combines with the Gα, forcing Gα dissociates with Gβ/Gγ dimer. The initial sequence Gα is used to pass the signal to the downstream.

加载失败时候的说明文字

Fig.1 The structure of Gα

Design

Use yeast Gα fusion to GPCR protein to make signal transduction, and we obtain the part by replicating the sequence from the yeast.

加载失败时候的说明文字

Fig.2 The schematic diagram of Modified Gα

Experiment

The part is used to transduct the signal that from the sweetness receptor to the downstream. The length of sequence is 1421bp.

加载失败时候的说明文字

Fig.3 The electrophoresis of the positive result of Gα.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]