Difference between revisions of "Part:BBa K2368007"
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<h1>Introduction</h1>
 
<h1>Introduction</h1>
 
<partinfo>BBa_K2368007 short</partinfo>
 
<partinfo>BBa_K2368007 short</partinfo>
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<p> This part consists of the promoter gal1/gal10, sweetness receptor T1R2 and the terminator CYC1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness receptor of the human tonge cells belonging to C type G protein coupled receptor.</p>
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<p>This part consists of the promoter gal1/gal10, sweetness receptor T1R2 and the terminator CYC1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness receptor of the human tonge cells belonging to C type G protein coupled receptor. </p>
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https://static.igem.org/mediawiki/2017/a/aa/G2C-1.png
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<p> Fig.1 The schematic diagram of Gal1/Gal10+T1R2+CYC1</p>
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[[File:T-BIT-China-2017parts-36.png|center|500px|默认文字]]
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<p style="text-align: center">Fig.1 The schematic diagram of Gal1/Gal10+T1R2+CYC1</p>
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<h1>Design</h1>
 
<h1>Design</h1>
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<p> We construct the part by Overlap Extension PCR. The Gal1/Gal10 promoter and CYC1 terminator stem from the PESC-Ura plasmid. We synthesize T1R2 by oligonucleotide synthesis.</p>
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<p>We construct the part by Overlap Extension PCR. The Gal1/Gal10 promoter and CYC1 terminator stem from the PESC-Ura plasmid. We synthesize T1R2 by oligonucleotide synthesis.</p>
 
<h1>Experiment</h1>
 
<h1>Experiment</h1>
 
<p> This part is used to express the sweetness receptor T1R2 in our experiment.</p>
 
<p> This part is used to express the sweetness receptor T1R2 in our experiment.</p>
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https://static.igem.org/mediawiki/2017/1/1f/G2C-2.png
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[[File:T-BIT-China-2017parts-37.png|center|500px|默认文字]]
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<p> Fig.2 Electrophoresis of Gal1/Gal10+T1R2+CYC1</p>
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<p style="text-align: center">Fig.2 Electrophoresis of Gal1/Gal10+T1R2+CYC1. </p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:53, 25 October 2017


Introduction

PGal 1/10-T1R2-CYC1t

This part consists of the promoter gal1/gal10, sweetness receptor T1R2 and the terminator CYC1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness receptor of the human tonge cells belonging to C type G protein coupled receptor.

默认文字

Fig.1 The schematic diagram of Gal1/Gal10+T1R2+CYC1



Design

We construct the part by Overlap Extension PCR. The Gal1/Gal10 promoter and CYC1 terminator stem from the PESC-Ura plasmid. We synthesize T1R2 by oligonucleotide synthesis.

Experiment

This part is used to express the sweetness receptor T1R2 in our experiment.

默认文字

Fig.2 Electrophoresis of Gal1/Gal10+T1R2+CYC1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
    Illegal BglII site found at 2061
    Illegal BglII site found at 2481
    Illegal BglII site found at 2643
    Illegal BamHI site found at 674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 1572
    Illegal AgeI site found at 2790
  • 1000
    COMPATIBLE WITH RFC[1000]