Difference between revisions of "Part:BBa K2368008"
Line 3: Line 3:
 
<h1>Introduction</h1>
 
<h1>Introduction</h1>
 
<partinfo>BBa_K2368008 short</partinfo>
 
<partinfo>BBa_K2368008 short</partinfo>
−
<p>This part consists of the promoter gal1/gal10, sweetness receptor T1R3 and the terminatorADH1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness receptor of the human tonge cells belonging to C type G protein coupled receptor.</p>
+
<p> This part consists of the promoter gal1/gal10, sweetness receptor T1R3 and the terminatorADH1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness receptor of the human tonge cells belonging to C type G protein coupled receptor. </p>
−
https://static.igem.org/mediawiki/2017/a/aa/G3A-1.png
+
 
−
<p> Fig.1 The schematic diagram of Gal1/Gal10+T1R3+ADH1</p>
+
[[File:T-BIT-China-2017parts-34.png|center|500px|默认文字]]
 +
<p style="text-align: center">Fig.1 The schematic diagram of Gal1/Gal10+T1R3+ADH1</p>
 +
 
 +
 
 +
 
 +
 
 
<h1>Design</h1>
 
<h1>Design</h1>
 
<p> We construct the part by Overlap Extension PCR. And the Gal1/Gal10 promoter and the ADH1 terminator stem from the PESC-Ura plasmid.</p>
 
<p> We construct the part by Overlap Extension PCR. And the Gal1/Gal10 promoter and the ADH1 terminator stem from the PESC-Ura plasmid.</p>
 
<h1>Experiment</h1>
 
<h1>Experiment</h1>
 
<p> This part is used to express the sweetness receptor T1R3 in our experiment.</p>
 
<p> This part is used to express the sweetness receptor T1R3 in our experiment.</p>
−
https://static.igem.org/mediawiki/2017/1/13/G3A-2.png
+
[[File:T-BIT-China-2017parts-35.png|center|500px|默认文字]]
−
<p> Fig.2 Electrophoresis of Gal1/Gal10+T1R3+ADH1.</p>
+
<p style="text-align: center">Fig.2 Electrophoresis of Gal1/Gal10+T1R3+ADH1.</p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 18: Line 23:
 
<!-- -->
 
<!-- -->
 
<h2>Sequence and Features</h2>
 
<h2>Sequence and Features</h2>
−
<partinfo>BBa_K2368008 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2368007 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
−
<partinfo>BBa_K2368008 arameters</partinfo>
+
<partinfo>BBa_K2368007 arameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 17:49, 25 October 2017


Introduction

PGal 1/10-T1R3-ADH1t

This part consists of the promoter gal1/gal10, sweetness receptor T1R3 and the terminatorADH1. The promoter gal1/gal10 is a bi-directional galactose-inducible promoter, which requires two percent of galactose to induce twelve hours to elicit protein expression. T1R3 is the sweetness receptor of the human tonge cells belonging to C type G protein coupled receptor.

默认文字

Fig.1 The schematic diagram of Gal1/Gal10+T1R3+ADH1



Design

We construct the part by Overlap Extension PCR. And the Gal1/Gal10 promoter and the ADH1 terminator stem from the PESC-Ura plasmid.

Experiment

This part is used to express the sweetness receptor T1R3 in our experiment.

默认文字

Fig.2 Electrophoresis of Gal1/Gal10+T1R3+ADH1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
    Illegal BglII site found at 2061
    Illegal BglII site found at 2481
    Illegal BglII site found at 2643
    Illegal BamHI site found at 674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 1572
    Illegal AgeI site found at 2790
  • 1000
    COMPATIBLE WITH RFC[1000]