Difference between revisions of "Part:BBa K2235009:Experience"
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===Characterizations of BBa_K2235009=== | ===Characterizations of BBa_K2235009=== | ||
'''Molecular cloning''' | '''Molecular cloning''' | ||
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Ligation of sialidase composite insert into pSB1C3 | Ligation of sialidase composite insert into pSB1C3 | ||
The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively. | The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively. | ||
Revision as of 17:48, 25 October 2017
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Characterizations of BBa_K2235009
Molecular cloning
Ligation of sialidase composite insert into pSB1C3 The gblock containing T7 promoter-RBS-sialidase was ligated into the Chloramphenicol plasmid backbone (pSB1C3). Preliminary confirmation of cloning was done by double digest Ecor1 and Pst1. Figure 1 represents the diggestion result: bands were visible at ~1600 bp and ~2000 bp which represents the insert and plasmid backbone respectively.
User Reviews
UNIQ55f65ce056b272ae-partinfo-00000000-QINU UNIQ55f65ce056b272ae-partinfo-00000001-QINU