Difference between revisions of "Part:BBa K2368014"
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<h1>Introduction</h1>
 
<h1>Introduction</h1>
 
<partinfo>BBa_K2368014 short</partinfo>
 
<partinfo>BBa_K2368014 short</partinfo>
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<p> This part is the overlap of that sweetness receptor T1R2 combines with the BCP tag. </p>
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<p>This part is the overlap of that sweetness receptor T1R2 combines with the BCP tag. </p>
 
<p> The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and BCP tag.</P>
 
<p> The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and BCP tag.</P>
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<P> The result of this part is that the expression of the blue cyanidin protein to detect the expression and the location of the T1R2.</p>
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<P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.</p>
 
<h1>Design</h1>
 
<h1>Design</h1>
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<p> Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and BCP. Then, we combined the overlap of T1R2 with the BCP by PCR.</p>
+
<p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and BCP. Then, we combined the overlap of T1R3 with the BCP by PCR.</p>
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https://static.igem.org/mediawiki/2017/a/af/BCP2-1.png
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[[File:T-BIT-China-2017parts-24.png|center|500px|默认文字]]
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<p>Fig.1 The schematic diagram of BCP+T1R2 overlap</p>
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<p style="text-align: center">Fig.1 The schematic diagram of BCP+T1R2 overlap</p>
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[[File:T-BIT-China-2017parts-25.png|center|500px|默认文字]]
 +
<p style="text-align: center">Fig. 2 The PCR method of BCP+T1R2 overlap</p>
 +
 
 
<h1>Experiment</h1>
 
<h1>Experiment</h1>
 
<p>This part is used to detect the expression and the location of the T1R2.</p>
 
<p>This part is used to detect the expression and the location of the T1R2.</p>
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https://static.igem.org/mediawiki/2017/0/0f/BCP2-2.png
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[[File:T-BIT-China-2017parts-26.png|center|500px|默认文字]]
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<p>Fig.2 Electrophoresis of BCP+T1R2 overlap</p>
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<p style="text-align: center">Fig.3 Electrophoresis of BCP+T1R2 overlap. Line one is the target</p>
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<h2>Sequence and Features</h2>
 
<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368014 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K2368013 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2368014 parameters</partinfo>
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<partinfo>BBa_K2368013 parameters</partinfo>
 
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Revision as of 17:25, 25 October 2017


Introduction

T1R2-BCP

This part is the overlap of that sweetness receptor T1R2 combines with the BCP tag.

The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and BCP tag.

The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.

Design

Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and BCP. Then, we combined the overlap of T1R3 with the BCP by PCR.

默认文字

Fig.1 The schematic diagram of BCP+T1R2 overlap

默认文字

Fig. 2 The PCR method of BCP+T1R2 overlap

Experiment

This part is used to detect the expression and the location of the T1R2.

默认文字

Fig.3 Electrophoresis of BCP+T1R2 overlap. Line one is the target


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]