Difference between revisions of "Part:BBa K2368012"
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<h1>Introduction</h1> | <h1>Introduction</h1> | ||
<partinfo>BBa_K2368012 short</partinfo> | <partinfo>BBa_K2368012 short</partinfo> | ||
| − | |||
<p> The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and YCP tag.</P> | <p> The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and YCP tag.</P> | ||
<P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.</p> | <P> The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.</p> | ||
<h1>Design</h1> | <h1>Design</h1> | ||
| − | <p> Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and YCP. Then, we combined the overlap of | + | <p>Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and YCP. Then, we combined the overlap of T1R3 with the YCP by PCR.</p> |
| − | + | [[File:T-BIT-China-2017parts-20.png|center|500px|默认文字]] | |
| − | <p>Fig. | + | <p style="text-align: center">Fig.1 The schematic diagram of YCP+T1R2 overlap</p> |
| + | [[File:T-BIT-China-2017parts-21.png|center|500px|默认文字]] | ||
| + | <p style="text-align: center">Fig. 2 The PCR method of YCP+T1R2 overlap</p> | ||
| + | |||
<h1>Experiment</h1> | <h1>Experiment</h1> | ||
<p>This part is used to detect the expression and the location of the T1R2.</p> | <p>This part is used to detect the expression and the location of the T1R2.</p> | ||
| − | + | [[File:T-BIT-China-2017parts-23.png|center|500px|默认文字]] | |
| − | <p>Fig. | + | <p style="text-align: center">Fig.3 Electrophoresis of YCP+T1R2 overlap. Line one is the target</p> |
| + | |||
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<h2>Sequence and Features</h2> | <h2>Sequence and Features</h2> | ||
| − | <partinfo> | + | <partinfo>BBa_K2368013 SequenceAndFeatures</partinfo> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
| − | <partinfo> | + | <partinfo>BBa_K2368013 parameters</partinfo> |
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Revision as of 17:17, 25 October 2017
Introduction
T1R2-YCP
The T1R2 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway. Also, it is necessary for us to show whether the T1R2 expressed correctly. As a result of that, we can perform the fusion between the N-terminal of T1R2 and YCP tag.
The result of this part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R2.
Design
Firstly, we constructed the specific primers that consists of the overlap regions of the T1R2 and YCP. Then, we combined the overlap of T1R3 with the YCP by PCR.
Fig.1 The schematic diagram of YCP+T1R2 overlap
Fig. 2 The PCR method of YCP+T1R2 overlap
Experiment
This part is used to detect the expression and the location of the T1R2.
Fig.3 Electrophoresis of YCP+T1R2 overlap. Line one is the target
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]