Difference between revisions of "Part:BBa K2440011"

Revision as of 16:52, 25 October 2017

let-7a target sequence

It is the target sequence of let-7a, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind let-7a in an Ago2 dependent manner, that is, knockdown of let-7a was achieved by transfecting cells with miRNA locker.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-MiRNA locker assembled by using this modularized DNA part, were able to bind let-7a in an Ago2 dependent manner, that is, knockdown of let-7a was achieved by transfecting cells with miRNA locker.
+MiRNA locker assembled by using this modularized DNA part was able to bind let-7a in an Ago2 dependent manner, that is, knockdown of let-7a was achieved by transfecting cells with miRNA locker.
 ===Sequence and Features===