Difference between revisions of "Part:BBa K2368004"
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<h1>Introduction</h1>
 
<h1>Introduction</h1>
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<partinfo>BBa_K2368003 short</partinfo>
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<p> The protein encoded by the T1R3 gene is a G protein-coupled receptor with seven trans-membrane domains. And it is a component of the heterodimeric amino acid taste receptor T1R1+3 and sweet taste receptor T1R2+3. This receptor is formed as a protein dimer with either T1R1 or T1R2. Experiments have also shown that a homo-dimer of T1R3 is also sensitive to natural sweeteners. This has been hypothesized as the mechanism by which sugar substitutes do not have the same taste qualities as natural sugars. </p>
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<p> The protein encoded by the T1R2 gene is a G protein-coupled receptor with seven trans-membrane domains and is a component of the heterodimeric amino acid taste receptor T1R1+3 and sweet taste receptor T1R2+3. This receptor is formed as a protein dimer with either T1R1 or T1R2. Experiments have also shown that a homo-dimer of T1R2 is also sensitive to natural sugar substances. This has been hypothesized as the mechanism by which sugar substitutes do not have the same taste qualities as natural sugars.</P>
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<p>Originally, T1R1+3 expressing cells are found in fungiform papillae at the tip and edges of the tongue and palate taste receptor cells in the roof of the mouth. These cells are shown to synapse upon the chorda tympani nerves to send their signals to the brain. T1R2+3 expressing cells are found in circumvallate papillae and foliate papillae near the back of the tongue and palate taste receptor cells in the roof of the mouth. These cells are shown to synapse upon the glossopharyngeal nerves to send their signals to the brain.</p>
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<P>Originally, T1R1+3 expressing cells are found in fungiform papillae at the tip and edges of the tongue and palate taste receptor cells in the roof of the mouth. These cells are shown to synapse upon the chorda tympani nerves to send their signals to the brain. T1R2+3 expressing cells are found in circumvallate papillae and foliate papillae near the back of the tongue and palate taste receptor cells in the roof of the mouth. These cells are shown to synapse upon the glossopharyngeal nerves to send their signals to the brain. </p>
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https://static.igem.org/mediawiki/2017/4/44/3-1.png
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<p> Fig.1  The gene location of T1R3 in human bodies</p>
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File:T-BIT-China-2017parts-11.png
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<h1>Design</h1>
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[[File:T-BIT-China-2017parts-11.png|center|500px|默认文字]]
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<p> And we synthesize the part by OE PCR based on oligo primers design, and the part is cloned into the plasmid pESC-URA by Gibson assembly.</p>
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<p style="text-align: center">Fig. 1 The schematic diagram of T1R2/T1R3 in cytomembrane</i></p>
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https://static.igem.org/mediawiki/2017/3/3c/3-2.png
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<p> Fig.2 The schematic diagram of T1R3-oligo </p>
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<h1>Experiment</h1>
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<p> In our experiment, this part is used to accept the signal inputted in our whole pathway. Last but not least, we construct the sweetness receptor successfully. </p>
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https://static.igem.org/mediawiki/2017/0/0d/3-3.png
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<p> Fig.3 The electrophoresis of the positive result of T1R3.</p>
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===Usage and Biology===
 
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<h2>Sequence and Features</h2>
 
<h2>Sequence and Features</h2>
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<partinfo>BBa_K2368003 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K2368004 parameters</partinfo>
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<partinfo>BBa_K2368003 parameters</partinfo>
 
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Revision as of 16:39, 25 October 2017


Introduction

T1R2

The protein encoded by the T1R2 gene is a G protein-coupled receptor with seven trans-membrane domains and is a component of the heterodimeric amino acid taste receptor T1R1+3 and sweet taste receptor T1R2+3. This receptor is formed as a protein dimer with either T1R1 or T1R2. Experiments have also shown that a homo-dimer of T1R2 is also sensitive to natural sugar substances. This has been hypothesized as the mechanism by which sugar substitutes do not have the same taste qualities as natural sugars.

Originally, T1R1+3 expressing cells are found in fungiform papillae at the tip and edges of the tongue and palate taste receptor cells in the roof of the mouth. These cells are shown to synapse upon the chorda tympani nerves to send their signals to the brain. T1R2+3 expressing cells are found in circumvallate papillae and foliate papillae near the back of the tongue and palate taste receptor cells in the roof of the mouth. These cells are shown to synapse upon the glossopharyngeal nerves to send their signals to the brain.

File:T-BIT-China-2017parts-11.png

默认文字

Fig. 1 The schematic diagram of T1R2/T1R3 in cytomembrane</i>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1369
    Illegal BglII site found at 1789
    Illegal BglII site found at 1951
    Illegal XhoI site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 880
    Illegal AgeI site found at 2098
  • 1000
    COMPATIBLE WITH RFC[1000]