Difference between revisions of "Part:BBa K2328027"
Jiangzhongyi (Talk | contribs) (→Results) |
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==Results== | ==Results== | ||
| − | ===Co-expression | + | ===Co-expression in E.coli BL21=== |
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| + | '''Fluorescence detection with Microplate Reader''' | ||
Plasmid pET28b with smURFP and HO-1 gene were transformed into E. coli BL-21. We used this induced bacteria to confirm the fluorescence and data showed a relatively high value, as shown in table 1. | Plasmid pET28b with smURFP and HO-1 gene were transformed into E. coli BL-21. We used this induced bacteria to confirm the fluorescence and data showed a relatively high value, as shown in table 1. | ||
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| + | '''Fluorescence imaging with FCFM ''' | ||
Then laser confocal microscopy was use to observe these bacteria, activate light of 640nm was used, as shown in Figure 2. | Then laser confocal microscopy was use to observe these bacteria, activate light of 640nm was used, as shown in Figure 2. | ||
Revision as of 16:24, 25 October 2017
smURFP I + Linker A + RBS I + HO-1 I
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage
Biology
Reference
Results
Co-expression in E.coli BL21
Fluorescence detection with Microplate Reader
Plasmid pET28b with smURFP and HO-1 gene were transformed into E. coli BL-21. We used this induced bacteria to confirm the fluorescence and data showed a relatively high value, as shown in table 1.
Table 1. Result of Microplate Reader in the black 96-well plate. Tube 1 and 2 are experimental group, and tube 3 is the control group.
Fluorescence imaging with FCFM
Then laser confocal microscopy was use to observe these bacteria, activate light of 640nm was used, as shown in Figure 2.
Figure 2. The result after induction, the upper one is the control group, and the inferior one is the experimental group.
For other experiments for some intestinal bacteria, we use our codon-optimized gene to express HO-1 in facultative anarobes.