Difference between revisions of "Part:BBa I742123"

Revision as of 19:08, 22 October 2007

Multi-host vector pTG262 converted to BioBrick vector

biobricked version of pTG262

Plasmid has a multi host replication origin and is competant in range of gram positive and gram negative bacteria.

More information about pTG262 may be found on the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Gene_Expression_In_Lactobacillus Edinburgh Yoghurt Wiki]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5543
Illegal NheI site found at 694
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 5549
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 5543
Illegal BglII site found at 3754
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 5543
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 5543
Plasmid lacks a suffix.
Illegal XbaI site found at 5558
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 3365
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal SapI.rc site found at 3578

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_I742123 short</partinfo>
@@ Line 6: / Line 5: @@
 Plasmid has a multi host replication origin and is competant in range of gram positive and gram negative bacteria.
+More information about pTG262 may be found on the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Gene_Expression_In_Lactobacillus Edinburgh Yoghurt Wiki]
 <!-- Add more about the biology of this part here