Difference between revisions of "Part:BBa K2244007"
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<partinfo>BBa_K2244007 short</partinfo> | <partinfo>BBa_K2244007 short</partinfo> | ||
| − | The device is a functional plasmid containing a MBC hydrolase gene (mheI) (https://parts.igem.org/Part:BBa_K2244004 BBa_K2244004), which encodes carbendazim hydrolase. In order to study expression of target gene in cytoplasm, mheI was fused to a reported gene mCherry (https://parts.igem.org/Part:BBa_J06504 BBa_J06504), which functions as indicator of the uptake of mheI. This part is regulated under the control of ColE promoter, which is from E. coli ColE1 plasmid. | + | The device is a functional plasmid containing a MBC hydrolase gene (mheI) ([https://parts.igem.org/Part:BBa_K2244004 BBa_K2244004]), which encodes carbendazim hydrolase. In order to study expression of target gene in cytoplasm, mheI was fused to a reported gene mCherry ([https://parts.igem.org/Part:BBa_J06504 BBa_J06504]), which functions as indicator of the uptake of mheI. This part is regulated under the control of ColE promoter, which is from E. coli ColE1 plasmid. |
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Revision as of 15:32, 25 October 2017
ColE promoter +mhei gene +mcherry gene +T1terminator
The device is a functional plasmid containing a MBC hydrolase gene (mheI) (BBa_K2244004), which encodes carbendazim hydrolase. In order to study expression of target gene in cytoplasm, mheI was fused to a reported gene mCherry (BBa_J06504), which functions as indicator of the uptake of mheI. This part is regulated under the control of ColE promoter, which is from E. coli ColE1 plasmid.
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Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1250
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1250
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 754
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1250
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1250
Illegal AgeI site found at 413 - 1000COMPATIBLE WITH RFC[1000]