Difference between revisions of "Part:BBa K2306010"

Revision as of 15:18, 25 October 2017

Cytosolic-abundant heat soluble protein 106094 "CAHS 106094" (T7 promoter, RBS and double terminator

This Biobrick features the gene sequence that encodes for the production of the tardigrade intrinsically disordered protein (TDP) "CAHS 106094". This is one of the several heat soluble proteins found in the tardigrades capable of protecting other fragile biological parts upon desiccation by generating a crystal matrix. Therefore, this tardigrade protein can be combined with other perishable biological material (e.g. proteins) to increase its stability after dehydration or even enable the possibility of stabilizing material with just a previous drying step. The sequence encoding for the gene is preceded by a T7 promoter (BBa_I712074) and an RBS (BBa_K2306014), which allows inducible overexpression of the protein with IPTG; and followed by a double terminator consisting of a rrnB T1 terminator and T7Te terminator (BBa_B0015).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2306010 short</partinfo>
-This Biobrick features the gene sequence that encodes for the production of the tardigrade intrinsically disordered protein (TDP) "CAHS 106094". This is one of the several heat soluble proteins found in the tardigrades capable of protecting other fragile biological parts upon desiccation by generating a crystal matrix. Therefore, this tardigrade protein can be combined with other perishable biological material (e.g. proteins) to increase its stability after dehydration. The sequence encoding for the gene is preceded by a T7 promoter (BBa_I712074) and an RBS, which allows inducible overexpression of the protein with IPTG; and followed by a double terminator consisting of a rrnB T1 terminator and T7Te terminator (BBa_B0015).
+This Biobrick features the gene sequence that encodes for the production of the tardigrade intrinsically disordered protein (TDP) "CAHS 106094". This is one of the several heat soluble proteins found in the tardigrades capable of protecting other fragile biological parts upon desiccation by generating a crystal matrix. Therefore, this tardigrade protein can be combined with other perishable biological material (e.g. proteins) to increase its stability after dehydration or even enable the possibility of stabilizing material with just a previous drying step. The sequence encoding for the gene is preceded by a T7 promoter (BBa_I712074) and an RBS (BBa_K2306014), which allows inducible overexpression of the protein with IPTG; and followed by a double terminator consisting of a rrnB T1 terminator and T7Te terminator (BBa_B0015).
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