Difference between revisions of "Part:BBa K2202000:Design"

 
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===Design Notes===
 
===Design Notes===
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The Sequence consists of an HIV reverse transcriptase binding site (HTBS) which form a 3' hairpin. One might need to use RNAaseA or H if they would like to remove this hairpin from the single-stranded DNA. To know more about design considerations check out:
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This sequence is modification of the parts BBa K2054001,BBa K2054002, BBa K2054003 ,BBa K2054004 , BBa K2054005  from the iGEM Hong University Team 2016 team. This year our team is produced a Pre-tetrahedron structure which folds upon binding to the
  
 
Elbaz, Johann, Peng Yin, and Christopher A. Voigt. "Genetic encoding of DNA nanostructures and their self-assembly in living bacteria." Nature Communications 7 (2016).
 
Elbaz, Johann, Peng Yin, and Christopher A. Voigt. "Genetic encoding of DNA nanostructures and their self-assembly in living bacteria." Nature Communications 7 (2016).
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===Source===
 
===Source===

Revision as of 15:15, 25 October 2017


ssDNA for producing in-vivo tetrahedral structure Strand1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 182
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101
    Illegal SapI site found at 272


Design Notes

This sequence is modification of the parts BBa K2054001,BBa K2054002, BBa K2054003 ,BBa K2054004 , BBa K2054005 from the iGEM Hong University Team 2016 team. This year our team is produced a Pre-tetrahedron structure which folds upon binding to the

Elbaz, Johann, Peng Yin, and Christopher A. Voigt. "Genetic encoding of DNA nanostructures and their self-assembly in living bacteria." Nature Communications 7 (2016).

Source

This Part was generated using a DNA nanotechnology software called Tiamat. This is a computationally designed sequence.

References