Difference between revisions of "Part:BBa K2332040"
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| − | This construct displays GFP fused with SpyTag on the cell surface of E. coli cells under the control of our blue light inducible promoter. Intimin' is a truncated version of Intimin (EaeA intimin), an outer membrane surface protein, which has shown to display proteins of similar size to GFP fused to its N terminal. This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display under blue light induction. | + | This construct displays GFP fused with SpyTag on the cell surface of E. coli cells under the control of our blue light inducible promoter. Intimin' is a truncated version of Intimin (EaeA intimin), an outer membrane surface protein, which has shown to display proteins of similar size to GFP fused to its N terminal. For blue-light transcriptional induction, cells must also express EL222 ([https://parts.igem.org/Part:BBa_K2332004 BBa_K2332004]). |
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| + | This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display under blue light induction. | ||
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Revision as of 13:50, 25 October 2017
Blue light inducible expression of Intimin'-GFP-SpyTag (Pblind Intimin'-GFP-SpyTag)
This construct displays GFP fused with SpyTag on the cell surface of E. coli cells under the control of our blue light inducible promoter. Intimin' is a truncated version of Intimin (EaeA intimin), an outer membrane surface protein, which has shown to display proteins of similar size to GFP fused to its N terminal. For blue-light transcriptional induction, cells must also express EL222 (BBa_K2332004).
This construct allows to directly observe through fluorescence microscopy the functionality of Intimin for cell surface display under blue light induction.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 2844
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 2844
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 2844
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 2844
Illegal NgoMIV site found at 880
Illegal NgoMIV site found at 1621 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2715