Difference between revisions of "Part:BBa K2332053"

Revision as of 13:48, 25 October 2017

Blue light inducible expression of GFP-SpyTag (Pblind GFP-SpyTag)

We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein upon blue-light (465nm) exposure. For blue-light transcriptional induction, cells must also express EL222 (BBa_K2332004).

To use the GFP-Spytag, one needs to transform E. Coli cells with our Biobrick and expose to blue-light to allow the cells to produce the fusion protein. Then, cells are lysed and the protein purified. It can then be used in combination with our SpyCatcher variants to visualise and quantify their binding affinity.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 723

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2332053 short</partinfo>
-We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein upon blue-light (465nm) exposure.
+We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein upon blue-light (465nm) exposure. For blue-light transcriptional induction, cells must also express EL222 ([https://parts.igem.org/Part:BBa_K2332004 BBa_K2332004]).
 To use the GFP-Spytag, one needs to transform E. Coli cells with our Biobrick and expose to blue-light to allow the cells to produce the fusion protein. Then, cells are lysed and the protein purified. It can then be used in combination with our SpyCatcher variants to visualise and quantify their binding affinity.