Difference between revisions of "Part:BBa K2492002:Experience"
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| − | Since we need to express protein in C.elegans, introns should be added to achieve a good expression level of protein. The part | + | Since we need to express protein in C.elegans, introns should be added to achieve a good expression level of protein. The part BBa_K2005050 is reporter mcherry(a kind of red fluorescence protein), we get the sequence of mcherry and add introns. |
The mcherry part we use is optimized for C.elegans and perform excellently under confocal microscope. The whole neuron with dendrite can be observed distinctly. We can even observe the branch of dendrite near the mouse. | The mcherry part we use is optimized for C.elegans and perform excellently under confocal microscope. The whole neuron with dendrite can be observed distinctly. We can even observe the branch of dendrite near the mouse. | ||
Revision as of 12:55, 25 October 2017
Since we need to express protein in C.elegans, introns should be added to achieve a good expression level of protein. The part BBa_K2005050 is reporter mcherry(a kind of red fluorescence protein), we get the sequence of mcherry and add introns.
The mcherry part we use is optimized for C.elegans and perform excellently under confocal microscope. The whole neuron with dendrite can be observed distinctly. We can even observe the branch of dendrite near the mouse.
Applications of BBa_K2492002
User Reviews
UNIQa4024aea1e2002ca-partinfo-00000000-QINU UNIQa4024aea1e2002ca-partinfo-00000001-QINU