Difference between revisions of "Part:BBa K2314106"
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<partinfo>BBa_K2314515 short</partinfo>
 
<partinfo>BBa_K2314515 short</partinfo>
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This part is a gene which encoding a cellobiose transporter(CDT-1).Assimilate cellobiose into intracellular.                                                                                                         
 
This part is a gene which encoding a cellobiose transporter(CDT-1).Assimilate cellobiose into intracellular.                                                                                                         
 
In our project,we contract an engineered strain to use cellobiose. We all know that S. cerevisiae cannot ferment the cellodextrins naturally released by cellulases and require cellulase cocktails supplemented by β-glucosidase to quantitatively produce fermentable glucose.
 
In our project,we contract an engineered strain to use cellobiose. We all know that S. cerevisiae cannot ferment the cellodextrins naturally released by cellulases and require cellulase cocktails supplemented by β-glucosidase to quantitatively produce fermentable glucose.

Revision as of 12:31, 25 October 2017


GH1-1 ( β-glucosidase) coding sequence

This part is a gene which encoding a cellobiose transporter(CDT-1).Assimilate cellobiose into intracellular. In our project,we contract an engineered strain to use cellobiose. We all know that S. cerevisiae cannot ferment the cellodextrins naturally released by cellulases and require cellulase cocktails supplemented by β-glucosidase to quantitatively produce fermentable glucose. Cellobiose is not catabolized by S. cerevisiae and is not accumulated in the cytoplasm.So we express a functional cellodextrin transport system(CDT-1) from N. crassa allow S. cerevisiae to grow with cellobiose as the sole carbon source. CDT-1 is high-affinity cellobiose transporters, with Michaelis constant (Km) values of 4.0T0.3mM, respectively. Cellobiose transport by CDT-1 is inhibited by excess cello-triose, and CDT-1 activity is also inhibited by cellotetraose. What’s more, the combinations of CDT-1 with GH1- 1 constitute fully functional cellodextrin transport systems. We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1115
    Illegal BamHI site found at 726
    Illegal XhoI site found at 322
    Illegal XhoI site found at 985
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 236
    Illegal BsaI site found at 376
    Illegal BsaI.rc site found at 1030
    Illegal SapI site found at 993
    Illegal SapI.rc site found at 1377