Difference between revisions of "Part:BBa K2314106"

 
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<partinfo>BBa_K2314515 short</partinfo>
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This part is a gene Which encoding &#946;-glucosidase.The &#946;-glucosidase capable of hydrolyzing cellobiose into glucose.                                                                              &#946;-glucosidase catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and oligosaccharides, with release of glucose. &#946;-glucosidases catalyze the hydrolysis of soluble cellodextrins to glucose and are a critical component of cellulase systems.In our project,we expressed the GH1-1and CDT-1 in EBY100,the combinations of CDT-1 with GH1- 1 constitute fully functional cellodextrin transport systems.We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2314515 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2314515 parameters</partinfo>
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Revision as of 12:23, 25 October 2017


GH1-1 ( β-glucosidase) coding sequence

This part is a gene Which encoding β-glucosidase.The β-glucosidase capable of hydrolyzing cellobiose into glucose. β-glucosidase catalyzes the hydrolysis of the glycosidic bonds to terminal non-reducing residues in beta-D-glucosides and oligosaccharides, with release of glucose. β-glucosidases catalyze the hydrolysis of soluble cellodextrins to glucose and are a critical component of cellulase systems.In our project,we expressed the GH1-1and CDT-1 in EBY100,the combinations of CDT-1 with GH1- 1 constitute fully functional cellodextrin transport systems.We have constructed an engineered bacterium that can be used as a carbon source, which can be used to produce ethanol by fermentation of fiber sugars, and the following are our experimental results.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1115
    Illegal BamHI site found at 726
    Illegal XhoI site found at 322
    Illegal XhoI site found at 985
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 236
    Illegal BsaI site found at 376
    Illegal BsaI.rc site found at 1030
    Illegal SapI site found at 993
    Illegal SapI.rc site found at 1377