Difference between revisions of "Part:BBa K2492000:Design"
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
Latest revision as of 12:09, 25 October 2017
GFP (optimised, for use in the C. elegans chassis)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 264
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 797
Design Notes
As far as we considered, GFP can well expressed in bacteria,however, eukaryote like C.elegans may not have good result with GFP without intron. Adding introns may easily solve this problem.
Source
GFP optimized with three introns