Difference between revisions of "Part:BBa K2350013"

Latest revision as of 11:50, 25 October 2017

PrbcL-CrtW-CrtZ-rrnB

This is a combination sequence that contains Crt-W. Crt-Z, a special design constitutive promoter, operon and a terminator. As the research of the pigment synthesis pathway, we can simply know that Crt-W and Crt-Z both need to be involved in this pathway to produce astaxanthin. And with a constitutive promoter, we can ensure that the gene will keep transcript and translate in-vivo so that the synthesis pathway through astaxanthin will keep inducing.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 420
Illegal BglII site found at 1160
Illegal BamHI site found at 1814
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2016

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2350013 short</partinfo>
-This is a combination sequence that contain Crt-W. Crt-Z, a special design constitutive promoter, operon and a terminator. As the research of the pigment synthesis pathway, we can simply know that Crt-W and Crt-Z both need to be involved in this pathway to produce astaxanthin. And with a constitutive promoter, we can ensure that the gene will keep transcript and translate in-vivo so that the synthesis pathway through astaxanthin will keep induce.
+This is a combination sequence that contains Crt-W. Crt-Z, a special design constitutive promoter, operon and a terminator. As the research of the pigment synthesis pathway, we can simply know that Crt-W and Crt-Z both need to be involved in this pathway to produce astaxanthin. And with a constitutive promoter, we can ensure that the gene will keep transcript and translate in-vivo so that the synthesis pathway through astaxanthin will keep inducing.
 <!-- Add more about the biology of this part here