Difference between revisions of "Part:BBa K2332053"
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We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein, which we experimentally used in combination with our Intimin-SpyCatcher Biobrick (see experiment). | We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein, which we experimentally used in combination with our Intimin-SpyCatcher Biobrick (see experiment). | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
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Revision as of 11:30, 25 October 2017
No part name specified with partinfo tag.
We designed our GFP-SpyTag Biobrick to test bacterial cell adhesion through fluorescence microscopy. This part produces the GFP-SpyTag fusion protein, which we experimentally used in combination with our Intimin-SpyCatcher Biobrick (see experiment).
To use the GFP-Spytag, one needs to transform E. Coli cells with our Biobrick and allow the cells to produce the fusion protein. Then, cells are lysed and the protein purified to be used in the experiment.
Sequence and Features No part name specified with partinfo tag.