Difference between revisions of "Part:BBa K2381010"
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<partinfo>BBa_K2381010 short</partinfo> | <partinfo>BBa_K2381010 short</partinfo> | ||
<p>In this part, the light-induced dimerization protein,pMag[https://parts.igem.org/Part:BBa_K2381000], is fused with the N fragment of split dCas9 through rigid linker.<br/> | <p>In this part, the light-induced dimerization protein,pMag[https://parts.igem.org/Part:BBa_K2381000], is fused with the N fragment of split dCas9 through rigid linker.<br/> | ||
− | The alpha-helix structure of the rigid linker | + | The alpha-helix structure of the rigid linker renders more cooperativity between split dCas9 and dimerization protein.<br/> |
− | + | Its protein sequence is A(EAAAK)3A, in which the flanking Ala guarantees a flexibility conjunction, which can decrease the effect on the function of protein. </p> | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 09:58, 25 October 2017
Cod1+ rigid linker+pMagFast
In this part, the light-induced dimerization protein,pMag[1], is fused with the N fragment of split dCas9 through rigid linker.
The alpha-helix structure of the rigid linker renders more cooperativity between split dCas9 and dimerization protein.
Its protein sequence is A(EAAAK)3A, in which the flanking Ala guarantees a flexibility conjunction, which can decrease the effect on the function of protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1099
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2277
- 1000COMPATIBLE WITH RFC[1000]