Difference between revisions of "Part:BBa K2381010"

 
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<partinfo>BBa_K2381010 short</partinfo>
 
<partinfo>BBa_K2381010 short</partinfo>
  
−
the linker is changed from flexibility to rigid amino acid
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
In this part, the light-induced dimerization protein,<a href="https://parts.igem.org/Part:BBa_K2381001">pMag</a>, is fused with the N fragment of split dCas9 through rigid linker.<br/>
 +
The alpha-helix structure of the rigid linker render more cooperativity between split dCas9 and dimerization protein.<br/>
 +
It's protein sequence is A(EAAAK)3A, the flanking Ala guarantees a flexibility conjunction, which will decrease the effect on the function of protein.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 09:48, 25 October 2017


Cod1+ rigid linker+pMagFast


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1099
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2277
  • 1000
    COMPATIBLE WITH RFC[1000]