Difference between revisions of "Part:BBa K2447013"
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<partinfo>BBa_K2447013 short</partinfo> | <partinfo>BBa_K2447013 short</partinfo> | ||
− | Tlpa36 proteins is constitutively produced, and depending on the temperature (if below 36 degree), it will form Tlpa36 dimers and capable of repressing the downstream promoter | + | Tlpa36 proteins is constitutively produced, and depending on the temperature (if below 36 degree), it will form Tlpa36 dimers and capable of repressing the downstream promoter pTlpa36; providing steric hindrance to prevent RNA polymerase to bind and elicit downstream expression. |
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Revision as of 09:15, 25 October 2017
Temperature-sensitive repressible system
Tlpa36 proteins is constitutively produced, and depending on the temperature (if below 36 degree), it will form Tlpa36 dimers and capable of repressing the downstream promoter pTlpa36; providing steric hindrance to prevent RNA polymerase to bind and elicit downstream expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 526
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 811
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1114
- 1000COMPATIBLE WITH RFC[1000]