Difference between revisions of "Part:BBa K2285015"

Revision as of 08:18, 25 October 2017

J23119+Target3+amilCP(RBS+amilCP+Term)

constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilCP(BBa_K1357009) as the reporter gene. The information of part amilCP was given at the original part page, consisting of an RBS, the blue chromoprotein amilCP, and a double terminator B0010+B0012.

Usage and Biology

During experiments, we found that expression of chromoproteins amilCP, amilGFP, eforRed and cjBlue took nearly 24 hours to see the colors with naked eyes on the LB plate. But they are still great reporters when it comes to build visualized systems which do not need to depend on extra equippments.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 constitutive promoter J23119 followed by a stem-loop structure Target3, with chromoprotein amilCP(BBa_K1357009) as the reporter gene. The information of part amilCP was given at the original part page, consisting of an RBS, the blue chromoprotein amilCP, and a double terminator B0010+B0012.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
 During experiments, we found that expression of chromoproteins amilCP, amilGFP, eforRed and cjBlue took nearly 24 hours to see the colors with naked eyes on the LB plate. But they are still great reporters when it comes to build visualized systems which do not need to depend on extra equippments.