Difference between revisions of "Part:BBa K2201207"
Line 14: Line 14:
 
TEXT
 
TEXT
  
−
[[File:T--Bielefeld-CeBiTec--YKE_AzoF_Alignment2.png|thumb|400px|center| <b>Figure 3:</b> Alignment of the protein sequence of the AzoF-RS and the M.janschii TyrRS.]]
+
[[File:T--Bielefeld-CeBiTec--YKE_AzoF_Alignment2.png|thumb|400px|center| <b>Figure 3:</b> Alignment of the protein sequence of the AzoF-RS and the <i>M. jannaschii</i> TyrRS.]]
  
 
TEXT
 
TEXT

Revision as of 07:33, 25 October 2017

Expression plasmid of an aminoacyl-tRNA synthetase for AzoPhe

aminoacyl-tRNA synthetase for incorporating AzoF at the amber codon

TEXT

Figure 1: Plasmid map of the BioBrick K2201207 coding for a AzoF-tRNA-synthetase system based on the ONBY-Part (K1416000). The ONBY-RS was removed by PCR and then a gene containing the AzoF-RS and matching overlaps sides was integrated through Gibson assembly.

TEXT

Figure 2: History of the plasmid construction of K2201207.

TEXT

Figure 3: Alignment of the protein sequence of the AzoF-RS and the M. jannaschii TyrRS.

TEXT



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1164
    Illegal BamHI site found at 1170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal NgoMIV site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 295
    Illegal SapI.rc site found at 327
    Illegal SapI.rc site found at 1120