Difference between revisions of "Part:BBa K2381001"

Revision as of 06:34, 25 October 2017

nMagHigh

nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms.
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts BBa_K2381017.
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 403

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2381001 short</partinfo>
-it is a light-induced dimerization protein
+nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms. <br/>
+This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts BBa_K2381017. <br/>
+If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
 <!-- Add more about the biology of this part here