Difference between revisions of "Part:BBa K2381000"

Revision as of 05:18, 25 October 2017

pMagFast

pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is NAD which is normal in nearly all organisms. This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It's function is proved by parts BBa_K2381017 If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.

Reference

Kawano, F., Suzuki, H., Furuya, A., & Sato, M. (2015). Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat Commun, 6, 6256. doi:10.1038/ncomms7256

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 87
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2381000 short</partinfo>
-pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. It's corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation. Their light sensing molecule is NAD which is normal in nearly all organisms.
+pMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52R/M55R. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52D/M55G. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is NAD which is normal in nearly all organisms.
 This part is pMag-Fast, whose dissociation rate is more rapid than the origin pMag. It's function is proved by parts BBa_K2381017
+If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
 <h3>Reference</h3>