Difference between revisions of "Part:BBa K2316001"

 
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<partinfo>BBa_K2316001 short</partinfo>
 
<partinfo>BBa_K2316001 short</partinfo>
  
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The parts is a triple truncation of dCas9 in HNH, Rec2, and RUVCIII2 domain with quick change to enhance dCas9 function. The truncation and quick change was based on the crystal structure of SpCas9. The protein product still functions as dCas9, which has abolished nuclease activity, but with more compact size.
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dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner. This is an improvement from part BBa_K2130001.
  
 
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Revision as of 04:44, 25 October 2017


∆RuvCIII-2 ∆HNH ∆REC2 Sp-dCas9 Enhanced

dCas9 is a catalytically inactive Cas9, which still retains it's ability to bind to DNA. For epigenetic regulation, the dCas9 is highly dependent upon the function of it's fusion partner. This is an improvement from part BBa_K2130001.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 804
    Illegal BamHI site found at 1622
    Illegal XhoI site found at 2578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2112
    Illegal BsaI site found at 2774
    Illegal BsaI.rc site found at 1027