Difference between revisions of "Part:BBa K2253001:Design"

 
 
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===Design Notes===
 
===Design Notes===
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Our lab uses the Lee/Dueber YTK Golden Gate standard, which, in terms of part overhangs, is different than the iGEM PhytoBrick standard. Thus, we had to redesign our P32 promoter sequence to contain the proper overhangs specified by the iGEM PhytoBrick standard.
 
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The sequence for the P32 promoter and RBS was obtained from Zhu et al. (2015) in their paper "Isolation of strong constitutive promoters from <i>Lactococcus lactis</i> subsp. Lactis N8." Our lab uses the Lee/Dueber YTK Golden Gate standard, which, in terms of part overhangs, is different than the iGEM PhytoBrick standard. Thus, we had to redesign our P32 promoter sequence to contain the proper overhangs specified by the iGEM PhytoBrick standard.
  
 
===Source===
 
===Source===
  
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The P32 promoter sequence was obtained from the genome of Lactoccocus lactis.  
+
The P32 promoter and its RBS sequence are natively found in the genome of <i>Lactococcus lactis</i>. Using the sequence information provided by Zhu et al. (2015), we ordered the promoter and RBS sequence as a gBlock from IDT.  
  
 
===References===
 
===References===
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 +
# Zhu, D. et al. Isolation of strong constitutive promoters from <i>Lactococcus lactis</i> subsp. Lactis N8. <i>FEMS Microbiol Lett</i>. 363(16): pii: fnv107 (2015).

Latest revision as of 03:19, 25 October 2017


Constitutive P32 promoter and RBS composite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence for the P32 promoter and RBS was obtained from Zhu et al. (2015) in their paper "Isolation of strong constitutive promoters from Lactococcus lactis subsp. Lactis N8." Our lab uses the Lee/Dueber YTK Golden Gate standard, which, in terms of part overhangs, is different than the iGEM PhytoBrick standard. Thus, we had to redesign our P32 promoter sequence to contain the proper overhangs specified by the iGEM PhytoBrick standard.

Source

The P32 promoter and its RBS sequence are natively found in the genome of Lactococcus lactis. Using the sequence information provided by Zhu et al. (2015), we ordered the promoter and RBS sequence as a gBlock from IDT.

References

  1. Zhu, D. et al. Isolation of strong constitutive promoters from Lactococcus lactis subsp. Lactis N8. FEMS Microbiol Lett. 363(16): pii: fnv107 (2015).