Difference between revisions of "Part:BBa K2253000"

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Constitutive P8 promoter and RBS composite

The P8 constitutive promoter is natively found Lactococcus lactis. Although the transcriptional efficiency of this promoter has been characterized and tested in Lactococcus lactis and other Gram-positive bacteria, its functionality in Gram-negative species such as E. coli has not been recorded in the literature. We have confirmed that E. coli transformed with a cassette plasmid containing the P8 promoter and E2-Crimson reporter is able to successfully express the E2-Crimson red fluorescent protein. As a PhytoBrick compatible part, P8 promoter can be assembled into a transcriptional unit via Golden Gate assembly method to upregulate expression of a gene of interest in E. coli as well as L. lactis. of a gene of interest in E. coli as well as L. lactis.

Sequence and Features

References

1. [1] Zhu, D. et al. Isolation of strong constitutive promoters from Lactococcus lactis subsp. Lactis N8. FEMS Microbiol Lett. 363(16): pii: fnv107 (2015).