Difference between revisions of "Part:BBa K2429036:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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The second HBG intron has a point mutation at bp 654 to match the intron used in a paper that used the HBG intron as part of their reporter.
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The second HBG intron has a point mutation at bp 654 from a C to an A because the paper we were referencing had mutated the HBG intron in the same way.
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===Source===
 
===Source===

Latest revision as of 23:41, 24 October 2017


phEF1a 3 Exon eYFP Reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3464
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3464
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3464
    Illegal BglII site found at 569
    Illegal BamHI site found at 1183
    Illegal BamHI site found at 2594
    Illegal XhoI site found at 968
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3464
    Illegal PstI site found at 315
    Illegal PstI site found at 820
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3464
    Illegal PstI site found at 315
    Illegal PstI site found at 820
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 81
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The second HBG intron has a point mutation at bp 654 from a C to an A because the paper we were referencing had mutated the HBG intron in the same way.

Source

The human beta globin introns came from the HEK cell genome. The sequences for YFP and BFP were obtained through BLAST, and the part comes from the genome of a jellyfish.


References