Difference between revisions of "Part:BBa K2450101"

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A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.
 
A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.
  
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<span class='h3bb'><b>Sequence and Features</b></span>
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=== Sequence and Features===
 
<partinfo>BBa_K2450101 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2450101 SequenceAndFeatures</partinfo>
  

Revision as of 20:42, 24 October 2017


TEV protease tagged with mCherry

A non-self-cleaving TEV protease sequence with an N terminal mCherry tag and a C terminal His tag. The fluorophore tag allows for relative quantification of expression. The His tag allows for purification using a nickel column.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1444
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

TEV protease is a well-characterised specific protease from the Tobacco Etch Virus, with a cleavage sequence of Glu-Asn-Leu-Tyr-Phe-Gln-(CUT)-Gly.

This is a non-self-cleaving protease as we wanted a constant level of TEV protease produced.

We have used TEV protease as both a substitute for cruzipain, another specific protease, and as a signal amplifier after simulated cruzipain cleavage.

This part does not require any other parts to be functional.