Difference between revisions of "Part:BBa K2450301"
(→Usage and Biology) |
|||
| Line 15: | Line 15: | ||
pTet is the promoter that can be bound by the Tet repressor, a dimer that is prevented from binding DNA by the presence of the antimicrobial tetracycline (ATC). TetR is a very tight-binding repressor, meaning that leakage is unlikely (in contrast to a system such as Lac). | pTet is the promoter that can be bound by the Tet repressor, a dimer that is prevented from binding DNA by the presence of the antimicrobial tetracycline (ATC). TetR is a very tight-binding repressor, meaning that leakage is unlikely (in contrast to a system such as Lac). | ||
| + | |||
| + | We have demonstrated that our part works as participated, by plate reader experiments measuring the amount of YFP fluorescence produced over time in different conditions. The results are detailed below. | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2450301 parameters</partinfo> | <partinfo>BBa_K2450301 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
Revision as of 20:30, 24 October 2017
pTet promoter coding eYFP
A pTet promoter and RBS encoding for YFP.
This part was designed to test the efficacy of a modified TetR (Tet repressor), BBa_K2450201. It is a medium strength pTet promotor.
This part will work as a reporter for a system relying on a change in the levels of TetR repression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
pTet is the promoter that can be bound by the Tet repressor, a dimer that is prevented from binding DNA by the presence of the antimicrobial tetracycline (ATC). TetR is a very tight-binding repressor, meaning that leakage is unlikely (in contrast to a system such as Lac).
We have demonstrated that our part works as participated, by plate reader experiments measuring the amount of YFP fluorescence produced over time in different conditions. The results are detailed below.