Difference between revisions of "Part:BBa K2201243"
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This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, <b>[https://parts.igem.org/Part:BBa_K1416004 BBa_K1416004]</b>) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.
 
This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, <b>[https://parts.igem.org/Part:BBa_K1416004 BBa_K1416004]</b>) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.
  
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<b>Important:</b> This part does not contain a promoter! For the functional part and the results, look up to <b>BBa_K2201343</b>.
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<b>Important:</b> This part does not contain a promoter! For the functional part and the results, look up to <b>[https://parts.igem.org/Part:BBa_K2201343 BBa_K2201343]</b>.
  
 
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Revision as of 19:46, 24 October 2017


Fusion protein of CFP and YFP with an amber codon in the linker

This part is an improved version of the aaRS-test system “pFRY” (a Reporter Plasmid for Measuring ncAA Incorporation, BBa_K1416004) of the [http://2014.igem.org/Team:Austin_Texas/photocage iGEM-Team Austin, Texas, 2014]. We investigated that there were some issues with the choice of RFP and GFP for the system and decided to improve the part by using CFP and YFP, for they form a FRET system which leads to a more accurate distinction between the partial (CFP) and the whole (CFP-YFP) expressed fusion protein.

Important: This part does not contain a promoter! For the functional part and the results, look up to BBa_K2201343.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 1420
  • 1000
    COMPATIBLE WITH RFC[1000]