Difference between revisions of "Part:BBa K2273000:Design"
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Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R (2016) <i>Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production.</i> Journal of Molecular Biology 362: 393-402. [https://www.ncbi.nlm.nih.gov/pubmed/?term=Substitution%20of%20the%20Native%20srfA%20Promoter%20by%20Constitutive%20Pveg%20in%20Two%20B.%20subtilis%20Strains%20and%20Evaluation%20of%20the%20Effect%20on%20Surfactin%20Production PubMed]<br><br> | Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R (2016) <i>Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production.</i> Journal of Molecular Biology 362: 393-402. [https://www.ncbi.nlm.nih.gov/pubmed/?term=Substitution%20of%20the%20Native%20srfA%20Promoter%20by%20Constitutive%20Pveg%20in%20Two%20B.%20subtilis%20Strains%20and%20Evaluation%20of%20the%20Effect%20on%20Surfactin%20Production PubMed]<br><br> | ||
− | Nakano MM, Xia LA, Zuber P (1991)<i>Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis.</i> J Bacteriol. 173(17):5487-93. | + | Nakano MM, Xia LA, Zuber P (1991) <i>Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis.</i> J Bacteriol. 173(17):5487-93. |
[https://www.ncbi.nlm.nih.gov/pubmed/?term=Transcription%20initiation%20region%20of%20the%20srfA%20operon,%20which%20is%20controlled%20by%20the%20comP-comA%20signal%20transduction%20system%20in%20Bacillus%20subtilis. PubMed]<br><br> | [https://www.ncbi.nlm.nih.gov/pubmed/?term=Transcription%20initiation%20region%20of%20the%20srfA%20operon,%20which%20is%20controlled%20by%20the%20comP-comA%20signal%20transduction%20system%20in%20Bacillus%20subtilis. PubMed]<br><br> | ||
Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) <i>The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs.</i> Nucleic Acids Res.44(5):2160-72. | Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) <i>The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs.</i> Nucleic Acids Res.44(5):2160-72. | ||
[https://www.ncbi.nlm.nih.gov/pubmed/?term=The%20quorum-sensing%20regulator%20ComA%20from%20Bacillus%20subtilis%20activates%20transcription%20using%20topologically%20distinct%20DNA%20motifs PubMed]<br><br> | [https://www.ncbi.nlm.nih.gov/pubmed/?term=The%20quorum-sensing%20regulator%20ComA%20from%20Bacillus%20subtilis%20activates%20transcription%20using%20topologically%20distinct%20DNA%20motifs PubMed]<br><br> | ||
Hamoen LW, Venema G, Kuipers OP (2003) <i>Controlling competence in Bacillus subtilis: shared use of regulators.</i> Microbiology. 149(Pt 1):9-17 [https://www.ncbi.nlm.nih.gov/pubmed/?term=Controlling+competence+in+Bacillus+subtilis%3A+shared+use+of+regulators PubMed]<br><br> | Hamoen LW, Venema G, Kuipers OP (2003) <i>Controlling competence in Bacillus subtilis: shared use of regulators.</i> Microbiology. 149(Pt 1):9-17 [https://www.ncbi.nlm.nih.gov/pubmed/?term=Controlling+competence+in+Bacillus+subtilis%3A+shared+use+of+regulators PubMed]<br><br> | ||
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Revision as of 17:48, 24 October 2017
PsrfA: a ComA-dependent promoter of Bacillus subtilis
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design
This part was generated with RFC10 Suffix and Prefix. It encodes for the srfA promoter that is part of the ComX quorum response of B. subtilis and was designed for the subproject "Communication" by the [http://2017.igem.org/Team:TU_Dresden iGEM Team TU Dresden 2017 (EncaBcillus - It's a trap!)].
Source
The srfA promoter of B. subtilis was amplified via PCR from the B. subtilis wild type W168 genome using the primers listed below.
PsrfA fwd RFC10 | gatcGAATTCGCGGCCGCTTCTAGAGTTGCGGCATCCCGCAAAAAATATTG |
PsrfA rev RFC10 | gatcCTGCAGCGGCCGCTACTAGTACTTTATAAGCAGTGAACATG |
References
Willenbacher J, Mohr T, Henkel M, Gebhard S, Mascher T, Syldatk C, Hausmann R (2016) Substitution of the native srfA promoter by constitutive Pveg in two B. subtilis strains and evaluation of the effect on Surfactin production. Journal of Molecular Biology 362: 393-402. PubMed
Nakano MM, Xia LA, Zuber P (1991) Transcription initiation region of the srfA operon, which is controlled by the comP-comA signal transduction system in Bacillus subtilis. J Bacteriol. 173(17):5487-93.
PubMed
Wolf D, Rippa V, Mobarec JC, Sauer P, Adlung L, Kolb P, Bischofs IB(2016) The quorum-sensing regulator ComA from Bacillus subtilis activates transcription using topologically distinct DNA motifs. Nucleic Acids Res.44(5):2160-72.
PubMed
Hamoen LW, Venema G, Kuipers OP (2003) Controlling competence in Bacillus subtilis: shared use of regulators. Microbiology. 149(Pt 1):9-17 PubMed