Difference between revisions of "Part:BBa K2302005:Experience"
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===Applications of BBa_K2302005=== | ===Applications of BBa_K2302005=== | ||
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| + | To confirm whether the pHisx6-PelB-CALB vector was expressed successfully, western blot was performed in this study. As showed in Fig. A, the expression level of CALB protein was markedly increased in bacteria with pHisx6-PelB-CALB vector and IPTG, as compared to control, indicating that the pHisx6-PelB-CALB vector was expressed successfully. Next, to further demonstrate whether the CALB can secrete to the medium, the supernatant was directly detected using western blot. The result found that CALB protein expression level was significantly induced by IPTG in pHisx6-PelB-CALB-transfected group. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 16:41, 24 October 2017
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Applications of BBa_K2302005
To confirm whether the pHisx6-PelB-CALB vector was expressed successfully, western blot was performed in this study. As showed in Fig. A, the expression level of CALB protein was markedly increased in bacteria with pHisx6-PelB-CALB vector and IPTG, as compared to control, indicating that the pHisx6-PelB-CALB vector was expressed successfully. Next, to further demonstrate whether the CALB can secrete to the medium, the supernatant was directly detected using western blot. The result found that CALB protein expression level was significantly induced by IPTG in pHisx6-PelB-CALB-transfected group.
User Reviews
UNIQ9cae5f84f9a5fe6f-partinfo-00000000-QINU UNIQ9cae5f84f9a5fe6f-partinfo-00000001-QINU