Difference between revisions of "Part:BBa K2267000"
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<h2>Explained </h2> | <h2>Explained </h2> | ||
Asr promoter belongs to asr gene encoding acid-shock RNA. It is a pH-responsive promoter native to E.coli which induces transcription in acidic environment from pH 4.0 ~ 5.0 , and shows low activity at a neutral pH. We chose asr promoter as the base of pH regulation system.https://static.igem.org/mediawiki/2017/8/8c/T--TUST_China--parts_Pasr.png | Asr promoter belongs to asr gene encoding acid-shock RNA. It is a pH-responsive promoter native to E.coli which induces transcription in acidic environment from pH 4.0 ~ 5.0 , and shows low activity at a neutral pH. We chose asr promoter as the base of pH regulation system.https://static.igem.org/mediawiki/2017/8/8c/T--TUST_China--parts_Pasr.png | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 16:24, 24 October 2017
a pH sensitive promoter Pasr
Explained
Asr promoter belongs to asr gene encoding acid-shock RNA. It is a pH-responsive promoter native to E.coli which induces transcription in acidic environment from pH 4.0 ~ 5.0 , and shows low activity at a neutral pH. We chose asr promoter as the base of pH regulation system.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 235
Illegal SpeI site found at 2 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 235
Illegal SpeI site found at 2
Illegal NotI site found at 9
Illegal NotI site found at 227 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 235
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 235
Illegal SpeI site found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 235
Illegal SpeI site found at 2 - 1000COMPATIBLE WITH RFC[1000]