Difference between revisions of "Part:BBa K2429017:Design"
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===Source=== | ===Source=== | ||
| − | The | + | The HBG intron came from HEK genome, and mKate from jellyfish. |
===References=== | ===References=== | ||
Revision as of 14:54, 24 October 2017
2 Exon mKate-HBG Reporter
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1582
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1582
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1582
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1582
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1582
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1487
Illegal SapI.rc site found at 55
Design Notes
This part is human-codon optimized. We found a BsaI site in the middle of mKate Exon 2, so we use site-directed mutagenesis to change the base pair at position 1450 from a G to an A. We also mutated the HBG Intron 2 at position 888 from a C to an A because the paper we were referencing had mutated the HBG intron in the same way.
Source
The HBG intron came from HEK genome, and mKate from jellyfish.