Difference between revisions of "Part:BBa K124002"

Revision as of 14:06, 24 October 2017

Yeast GPD (TDH3) Promoter

Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Group: Tel-Hai 2017 Author: Yaakov Bulka

This is a strong constitutive yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase. Also called TDH3 or GAPDH. Studies have found the promoter activity of TDH3 decreased significantly when glycerol or xylose was supplied as the carbon source and at high temperature (42 °C) . Oxygen conditions had non-significant effect.

About the protein - the promoter was taken from the Glyceraldehyde-3-phosphate dehydrogenase peptide (GAPDH), that involves in glycolysis and gluconeogenesis. The protein is a tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate, detected in the cytoplasm and cell wall. GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria.

http://onlinelibrary.wiley.com/doi/10.1002/yea.1806/full#bib17

https://www.ncbi.nlm.nih.gov/pubmed/26164057

@@ Line 13: / Line 13: @@
 Group: Tel-Hai 2017
-Author: (the authors of the improvement)
+Author: Yaakov Bulka
-Summary: (a 1-3 sentence summary of your improvement)
-the documentation of your improvement (text, graphs, pictures, tables, etc.)
-Include sections for purpose, results, methods, etc. (if applicable)
-Uploads: (links to uploads relevant to your contribution, ex: csv containing your data, sequence files, etc.) Note: All files pertaining to a contribution must be uploaded to the Registry.
-Regulatory region spanning 680 bp upstream of the start codon of the GPD1 gene in yeast.