Difference between revisions of "Part:BBa K2350009"
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<partinfo>BBa_K2350009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2350009 SequenceAndFeatures</partinfo> |
Revision as of 13:55, 24 October 2017
Pigment double-crossover homologous gene recombination bacbone (pPIGBACK)
The vector, pPIGBACK, is used to transform into S. elongatus PCC 7942 with the inserted pigment gene in our project. pPIGBACK contains 5’- and 3’-ends of the neutral site II (NSII), replication origin of pBR322, ampicillin resistance gene, and double terminator BBa_B0015. The strategy we chose to construct the vector is to fuse B0015 and AmpR together first. Secondly, we fused 5’- and 3’-ends of the neutral site II (NSII) with PBR322 replication origin (ORI) together. At last, we ligated two parts together. The aim of constructing this vector is to finish double-crossover homologous gene recombination in S. elongatus PCC 7942. To insert BBa_J04450 with EcoR1 and Pst1 cutting sites, we use site-directed mutagenesis to remove all Pst1 cutting sites in pPIGBACK.
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found at 1
Illegal suffix found at 3880 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1
Illegal SpeI site found at 3881
Illegal PstI site found at 3895
Illegal NotI site found at 7
Illegal NotI site found at 1321
Illegal NotI site found at 3888 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 1
Illegal BglII site found at 1883
Illegal BamHI site found at 2180
Illegal XhoI site found at 1118 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 1
Illegal suffix found at 3881 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 1
Plasmid lacks a suffix.
Illegal XbaI site found at 16
Illegal SpeI site found at 3881
Illegal PstI site found at 3895
Illegal NgoMIV site found at 2101 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 972
Illegal BsaI site found at 1783