Difference between revisions of "Part:BBa K2350022:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We used fusion PCR to fuse CrtZ and PrbcL | + | We used fusion PCR to fuse CrtZ and PrbcL to avoid restriction site in between which might influence expression efficiency. |
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===Source=== | ===Source=== | ||
Revision as of 06:49, 24 October 2017
pPIGBACK-PrbcL-CrtZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used fusion PCR to fuse CrtZ and PrbcL to avoid restriction site in between which might influence expression efficiency.
Source
pPIGBACK 5'3'NSII and PrbcL are from Synechococcus elongatus PCC7942. pPIGBACK ori and ampicillin resistant gene are from pBR322. CrtZ is from Pantoea ananatis.