Revision as of 03:04, 24 October 2017

Introduction

Myc+T1R2 overlap

This part sweetness receptor T1R2 fusion with the MYC tag, just like the picture showed below.

Fig.1 The schematic diagram of MYC+T1R2 overlap

Design

In order to show whether the sweet receptor T1R2 are translated and located correctly, we fusion the MYC tag to N-terminal of T1R2. The reason fused tag to N-terminal is that the C-terminal of T1R2 interacts with the G protein α subunit, which is a crucial factor during signal deliver through G protein signal pathway. And the N-terminal is outside the cell, so it can be detected without killing it, using immunofluorescence can figure out whether T1R2 is located at the right position.

Fig.2 Electrophoresis of MYC+T1R2 overlap.

Experiment

At the beginning, we construct the specific primer that consists of the gene sequence of MYC tag. Then, fused T1R2 with the MYC using PCR. The length of sequence is 517bp.

Sequence and Features