Difference between revisions of "Part:BBa K2381016"

 
 
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<partinfo>BBa_K2381016 short</partinfo>
 
<partinfo>BBa_K2381016 short</partinfo>
  
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Insert the gRNA sequence behind the PcpcG2, the promoter corresponding to CcaS-CcaR system. The transcription of gRNA will be under the control of light.
+
the light-activated conventional split dCas9 fused split dCas9 with light-induced dimerization protein and can be activated under the irritation of blue light.
  
 
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<!-- Add more about the biology of this part here

Latest revision as of 02:43, 24 October 2017


light-activated conventional split dCas9 generator

the light-activated conventional split dCas9 fused split dCas9 with light-induced dimerization protein and can be activated under the irritation of blue light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1179
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3133