Difference between revisions of "Part:BBa K2491013:Experience"
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===Applications of BBa_K2491013=== | ===Applications of BBa_K2491013=== | ||
Degradation of Fluorene | Degradation of Fluorene | ||
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| + | The data show all absorbance measurements obtained during the biotransformation of phenanthrene and fluorene by our recombinant E.coli in minimal medium supplemented with PAHs. To evaluate whether the recombinant cells had the ability to transform PAHs, growth experiments were set up with various clones expressing the fluorene or phenanthrene catabolic pathway. The clones described above with the catabolic pathway under the control of 3 different constitutive were set in cultures using minimal medium supplemented with fluorene (0.1 mg/mL) and phenanthrene (0.1 mg/mL) as sole source of carbon (figures below). Antibiotics were added as appropriately. | ||
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| + | Clones containing the catabolic pathway exhibited higher cell density at 48 hours compared to their respective controls (vector alone) with the strongest promoter given a greater advantage (clone 48 for fluorene and clone 57 for phenanthrene). PAHs dissolved in DMSO appeared slightly more available than PAHs dissolved in methanol. This may be explained by the fact that PAHs stock solutions prepared in methanol exhibited some precipitates not observed with DMSO-based stocks. | ||
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| + | <img src="pic as graph.png" alt="Graph of Minimal Media"> | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 21:06, 23 October 2017
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Applications of BBa_K2491013
Degradation of Fluorene
The data show all absorbance measurements obtained during the biotransformation of phenanthrene and fluorene by our recombinant E.coli in minimal medium supplemented with PAHs. To evaluate whether the recombinant cells had the ability to transform PAHs, growth experiments were set up with various clones expressing the fluorene or phenanthrene catabolic pathway. The clones described above with the catabolic pathway under the control of 3 different constitutive were set in cultures using minimal medium supplemented with fluorene (0.1 mg/mL) and phenanthrene (0.1 mg/mL) as sole source of carbon (figures below). Antibiotics were added as appropriately.
Clones containing the catabolic pathway exhibited higher cell density at 48 hours compared to their respective controls (vector alone) with the strongest promoter given a greater advantage (clone 48 for fluorene and clone 57 for phenanthrene). PAHs dissolved in DMSO appeared slightly more available than PAHs dissolved in methanol. This may be explained by the fact that PAHs stock solutions prepared in methanol exhibited some precipitates not observed with DMSO-based stocks.
<img src="pic as graph.png" alt="Graph of Minimal Media">
User Reviews
UNIQ3eb70ee024f84ea4-partinfo-00000000-QINU UNIQ3eb70ee024f84ea4-partinfo-00000001-QINU