Difference between revisions of "Part:BBa K2429017"
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This part splits the red fluorescent mKate reporter into two exons, with human beta globin (HBG) intron 2 in between these two exons. We used this split mKate 2-exon construct as a reporter construct that we used to determine if our dCas13 or Ms2 systems were successful in splicing out the HBG Intron 2. We used HBG Intron 2 because it is a standard intron used in reporter constructs. | This part splits the red fluorescent mKate reporter into two exons, with human beta globin (HBG) intron 2 in between these two exons. We used this split mKate 2-exon construct as a reporter construct that we used to determine if our dCas13 or Ms2 systems were successful in splicing out the HBG Intron 2. We used HBG Intron 2 because it is a standard intron used in reporter constructs. | ||
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| + | <img src="https://static.igem.org/mediawiki/parts/0/03/2ex.png" alt="2 Exon reporter"> | ||
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Revision as of 21:03, 23 October 2017
2 Exon mKate-HBG Reporter
This part splits the red fluorescent mKate reporter into two exons, with human beta globin (HBG) intron 2 in between these two exons. We used this split mKate 2-exon construct as a reporter construct that we used to determine if our dCas13 or Ms2 systems were successful in splicing out the HBG Intron 2. We used HBG Intron 2 because it is a standard intron used in reporter constructs.
<img src="
" alt="2 Exon reporter">
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1582
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1582
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1582
Illegal BamHI site found at 1 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1582
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1582
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1487
Illegal SapI.rc site found at 55